ORGANISATION/COMPANYJohannes Kepler University
RESEARCH FIELDBiological sciences › Biological engineeringPhysics › Biophysics
RESEARCHER PROFILEFirst Stage Researcher (R1)
APPLICATION DEADLINE24/11/2019 23:00 - Europe/Brussels
LOCATIONAustria › Linz
TYPE OF CONTRACTTemporary
HOURS PER WEEK40
OFFER STARTING DATE01/12/2019
EU RESEARCH FRAMEWORK PROGRAMMEH2020 / Marie Skłodowska-Curie Actions
MARIE CURIE GRANT AGREEMENT NUMBER860592
PhD student in Biophysics/Molecular Biology (3 years)
The position is part of the Innovative Training Network (ITN) “PROTON”. The network is devoted to the investigation of proton transport and proton-coupled transport. It is funded under Marie Sklodowska-Curie actions (H2020-MSCA-ITN-2019, grant agreement number: 860592).
The successful applicant will perform her/his studies and research at the
Institute of Biophysics (Division of Molecular and Membrane Biophysics) at Johannes Kepler Universität Linz, Austria, She/he will join the Membrane Transport Group (Head Peter Pohl) that is mainly interested in spontaneous and facilitated transport of water, protons and other small molecules as well as in membrane protein translocation across the reconstituted translocation channel. Preferentially we use electrophysiology, fluorescence correlation spectroscopy, light scattering (in stopped flow mode) and electrochemical microscopy.
These will be your tasks
The successful applicant will investigate proton’s ability to migrate along the membrane surface. The process is important to bioenergetics, since it ensures the most efficient coupling between mitochondrial proton pumps and ATPases. The scope of this research project is to understand the compositional and geometrical requirements that the membrane surface must fulfil for its waters of hydration to generate these forces.
The successful applicant will photo-release protons from caged compounds that are either adsorbed to one of the membrane leaflets or dissolved in the surrounding aqueous bulk solution and monitor their arrival at the other side of the membrane by using pH-sensitive dyes at the exit tunnel of reconstituted proton channels or carriers. As facilitators of proton transport, we will use recombinant purified proteins or artificial proton channels. The facilitator will be modified by site directed mutagenesis or chemical synthesis, respectively, to elucidate the role of titratable residues at the channel mouth.
The PhD student will have two secondments (2 to 3 months each):
- Kiel University, Germany (Eric Beitz); in vitro protein expression
- Carl Zeiss Jena, Germany (Klaus Weisshart; High resolution single molecule microscopy
You're offering these qualifications
You have a master degree in Physics, Biophysics, Biology, Biochemistry or a comparable qualification.
We're offering these benefits
We are offering a full time position for three years. The gross salary amounts to about €40,000 per year (corresponds to roughly €1900 x 14 (12 months + 2 additional payments) after taxes and social security). A monthly mobility allowance and - if applicable - a family allowance will be paid in addition.
- Motivation letter
- Reference letters
- Copies of Bachelor and Master certificates
- Publications (if available).
- Applicants must, at the date of recruitment by PROTON, be in the first four years (full-time equivalent research experience measured from the date when the researcher obtained the degree entitling him/her to embark on a doctorate) of their research careers
- They should not have been awarded a doctoral degree.
- Applicants must not have resided or carried out their main activity (work, studies, etc.) in the country of the recruiting institution for more than 12 months in the 3 years immediately before the recruitment date. This excludes short stays such as holidays or compulsory national service.
- We look for students with exceptional academic performance holding or very close to completing their Master degree in Physics, Chemistry, Biophysics, Biochemistry or closely related subjects,
- Knowledge of the English language is essential.
- Knowledge of light microscopic and spectroscopic techniques (e.g. confocal microscopy, fluorescence correlation spectroscopy, single dye tracing)
- Experience in handling fluorescent molecules, quantum dots, etc.
- Publications in international journals/attendance of international conferences in the following fields: optics, surface chemistry, biophysics.
- Site directed mutagenesis, protein overexpression
- Protein purification and membrane reconstitution
- Ability to use programming languages for data acquisition
- Soft skills: presentation skill, team working ability
For all the above, knowledge is proven by publications, certificates, master thesis or equivalent documentation. Skills should be acknowledged in reference letters and demonstrated during the interview (presentation skills). During the interview each applicants presents his/her master thesis and a scientific paper from an area unrelated to his/her specialization (chosen by the interviewers).
Women are encouraged to apply. The recruitment committee gives preference to female candidates if they are equally qualified as male candidates.
EURAXESS offer ID: 437625
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